Characterisation of Vasodilator-Stimulated Phosphoprotein DdVASP, a third profilin isoform and Ste-20 like kinases from Dictyostelium discoideum
نویسندگان
چکیده
Diese Dissertation wurde selbständig und ohne unerlaubte Hilfsmittel angefertigt. (2005). The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia. (2006). Characterisation of the Ste20-like kinase Krs1 of Dictyostelium discoideum. Eur. (2003) STE20-like kinases and their role in the actin cytoskeleton in Dictyostelium discoideum. The Ste20-like kinase DST10 is involved in phagocytosis and late development of Dictyostelium discoideum. Eur. J. Cell Biology 84 Abstract: O-23 The experimental parts of the work presented here were carried out in the laboratory of
منابع مشابه
Profilin isoforms in Dictyostelium discoideum.
Eukaryotic cells contain a large number of actin binding proteins of different functions, locations and concentrations. They bind either to monomeric actin (G-actin) or to actin filaments (F-actin) and thus regulate the dynamic rearrangement of the actin cytoskeleton. The Dictyostelium discoideum genome harbors representatives of all G-actin binding proteins including actobindin, twinfilin, and...
متن کاملRequirement of a vasodilator-stimulated phosphoprotein family member for cell adhesion, the formation of filopodia, and chemotaxis in dictyostelium.
We have examined the function of a member of the vasodilator-stimulated phosphoprotein family of proteins (DdVASP) in Dictyostelium. Ddvasp null cells lack filopodia, whereas targeting DdVASP to the plasma membrane with a myristoyl tag results in a significant increase in filopodia. The proline-rich domain-Ena/VASP homology 2 structure is required for both actin polymerization activity and filo...
متن کاملThe bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation.
Filopodia are highly dynamic finger-like cell protrusions filled with parallel bundles of actin filaments. Previously we have shown that Diaphanous-related formin dDia2 is involved in the formation of filopodia. Another key player for the formation of filopodia across many species is vasodilator-stimulated phosphoprotein (VASP). It has been proposed that the essential role of VASP for formation...
متن کاملThe mammalian profilin isoforms display complementary affinities for PIP2 and proline-rich sequences.
We present a study on the binding properties of the bovine profilin isoforms to both phosphatidylinositol 4,5-bisphosphate (PIP2) and proline-rich peptides derived from vasodilator-stimulated phosphoprotein (VASP) and cyclase-associated protein (CAP). Using microfiltration, we show that compared with profilin II, profilin I has a higher affinity for PIP2. On the other hand, fluorescence spectro...
متن کاملIdentification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein).
VASP (vasodilator-stimulated phosphoprotein), an established substrate of cAMP- and cGMP-dependent protein kinases in vitro and in living cells, is associated with focal adhesions, microfilaments, and membrane regions of high dynamic activity. Here, the identification of an 83-kDa protein (p83) that specifically binds VASP in blot overlays of different cell homogenates is reported. With VASP ov...
متن کامل